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Test Name REF LAB EGFR MUTATION ANALYSIS
Orderable CPT -
OVT 123002545
Synonyms -
Result Test Name QUEST SPECIMEN TYPE/SOURCE
Laboratory QUEST LAB
Result Test Code 123924765
Laboratory Test Name QUEST EGFR MUTATION ANALYSIS
Reportable Test Name -
Result LOINC 31208-2
Collection Container PATH CONTAINER
Units -
Collection Requirements Preferred Specimen(s) Formalin-fixed paraffin-embedded tissue block Alternative Specimen(s) 8 unstained charged (+) slides Minimum Volume 4 unstained charged (+) slides Collection Instructions FFPE tissue block: Regular procedure for paraffin-embedded tissue block preparation. Slide preparation: Cut one 3-5 microns tissue section for H&E stain. Cut 3-5 (10 microns) sections on charged regular glass slides for macrodissection. The slide should not be baked or melted. A new blade must be used for cutting each paraffin block to prevent cross contamination. Minimum volume: 3x10 um sections; 1x4 um section with H&E stain Standard volume: 5x10 um sections; 1x4 um section with H&E stain Do not reject specimens. Send to laboratory for screening.
Container Temp Room Temperature (1)
Container Volume -
Test Info Epidermal Growth Factor Receptor (EGFR) Mutation Analysis - EGFR kinase domain mutations are found in 10-15% of lung adenocarcinomas particularly those occurring in younger patients without a smoking history and identify tumors that may have significant clinical response to EGFR-targeted kinase inhibitors. PCR-based DNA sequencing is used to assess for mutations in exons 18-21 of EGFR which are the sites of greater than 95% of somatic mutations observed in tumors. This assay will also detect the T790M mutation which is commonly seen in acquired resistance. With this information physicians will be able to select those patients who are most likely to respond to targeted lung cancer therapy. In addition follow-up samples from patients with resistant or recurrent disease may demonstrate an acquired T790M mutation where newer FDA-approved agents have been shown to be effective.
Shipping Instructions Transport Container Formalin-fixed paraffin-embedded tissue block Transport Temperature Room temperature Specimen Stability Room temperature: 5 years Refrigerated: 5 years Frozen: Unacceptable Reject Criteria Hemolysis Lipemia
Result Test Name QUEST EGFR MUTATION ANALYSIS
Laboratory QUEST LAB
Result Test Code 123924767
Laboratory Test Name QUEST EGFR MUTATION ANALYSIS
Reportable Test Name -
Result LOINC 21665-5
Collection Container PATH CONTAINER
Units -
Collection Requirements Preferred Specimen(s) Formalin-fixed paraffin-embedded tissue block Alternative Specimen(s) 8 unstained charged (+) slides Minimum Volume 4 unstained charged (+) slides Collection Instructions FFPE tissue block: Regular procedure for paraffin-embedded tissue block preparation. Slide preparation: Cut one 3-5 microns tissue section for H&E stain. Cut 3-5 (10 microns) sections on charged regular glass slides for macrodissection. The slide should not be baked or melted. A new blade must be used for cutting each paraffin block to prevent cross contamination. Minimum volume: 3x10 um sections; 1x4 um section with H&E stain Standard volume: 5x10 um sections; 1x4 um section with H&E stain Do not reject specimens. Send to laboratory for screening.
Container Temp Room Temperature (1)
Container Volume -
Test Info Epidermal Growth Factor Receptor (EGFR) Mutation Analysis - EGFR kinase domain mutations are found in 10-15% of lung adenocarcinomas particularly those occurring in younger patients without a smoking history and identify tumors that may have significant clinical response to EGFR-targeted kinase inhibitors. PCR-based DNA sequencing is used to assess for mutations in exons 18-21 of EGFR which are the sites of greater than 95% of somatic mutations observed in tumors. This assay will also detect the T790M mutation which is commonly seen in acquired resistance. With this information physicians will be able to select those patients who are most likely to respond to targeted lung cancer therapy. In addition follow-up samples from patients with resistant or recurrent disease may demonstrate an acquired T790M mutation where newer FDA-approved agents have been shown to be effective.
Shipping Instructions Transport Container Formalin-fixed paraffin-embedded tissue block Transport Temperature Room temperature Specimen Stability Room temperature: 5 years Refrigerated: 5 years Frozen: Unacceptable Reject Criteria Hemolysis Lipemia
Result Test Name BLOCK/SPECIMEN ID
Laboratory QUEST LAB
Result Test Code 123013765
Laboratory Test Name QUEST EGFR MUTATION ANALYSIS
Reportable Test Name -
Result LOINC -
Collection Container PATH CONTAINER
Units -
Collection Requirements Preferred Specimen(s) Formalin-fixed paraffin-embedded tissue block Alternative Specimen(s) 8 unstained charged (+) slides Minimum Volume 4 unstained charged (+) slides Collection Instructions FFPE tissue block: Regular procedure for paraffin-embedded tissue block preparation. Slide preparation: Cut one 3-5 microns tissue section for H&E stain. Cut 3-5 (10 microns) sections on charged regular glass slides for macrodissection. The slide should not be baked or melted. A new blade must be used for cutting each paraffin block to prevent cross contamination. Minimum volume: 3x10 um sections; 1x4 um section with H&E stain Standard volume: 5x10 um sections; 1x4 um section with H&E stain Do not reject specimens. Send to laboratory for screening.
Container Temp Room Temperature (1)
Container Volume -
Test Info Epidermal Growth Factor Receptor (EGFR) Mutation Analysis - EGFR kinase domain mutations are found in 10-15% of lung adenocarcinomas particularly those occurring in younger patients without a smoking history and identify tumors that may have significant clinical response to EGFR-targeted kinase inhibitors. PCR-based DNA sequencing is used to assess for mutations in exons 18-21 of EGFR which are the sites of greater than 95% of somatic mutations observed in tumors. This assay will also detect the T790M mutation which is commonly seen in acquired resistance. With this information physicians will be able to select those patients who are most likely to respond to targeted lung cancer therapy. In addition follow-up samples from patients with resistant or recurrent disease may demonstrate an acquired T790M mutation where newer FDA-approved agents have been shown to be effective.
Shipping Instructions Transport Container Formalin-fixed paraffin-embedded tissue block Transport Temperature Room temperature Specimen Stability Room temperature: 5 years Refrigerated: 5 years Frozen: Unacceptable Reject Criteria Hemolysis Lipemia