Result Test Name
QUEST BRAF V600 MUTATION
Laboratory
QUEST LAB
Result Test Code
123924728
Laboratory Test Name
QUEST MELANOMA BRAF V600 MUTATION COBAS
Reportable Test Name
-
Result LOINC
58415-1
Collection Container
PATH CONTAINER
Units
-
Collection Requirements
Preferred Specimen(s) Formalin-fixed paraffin embedded tissue Alternative Specimen(s) Slides accepted only after contacting Oncology Center as listed in instructions Collection Instructions Slides: Please contact the Oncology Center at 1(800)642-4657 x2906 prior to submission of slides for detailed instructions. Freezing is not recommended for FFPE.
Container Temp
Room Temperature (1)
Container Volume
-
Test Info
Melanoma BRAF V600 Mutation Cobas? - This FDA-cleared real-time PCR assay on the cobas 4800 platform assesses for mutations at codon 600 of BRAF particularly V600E which are present in 40-60% of melanomas. Melanomas with codon 600 BRAF mutations are associated with response to the BRAF-directed kinase inhibitor vemurafenib (ZELBORAF?). Tumor DNA is extracted from fixed tumor tissue and subjected to real-time PCR with approximate sensitivity of 5% mutation-bearing cells in a mixed sample. Other codon 600 BRAF mutations beside the most common V600E (c.1799T>A) may not be detected by this method.
Shipping Instructions
Transport Container Formalin-fixed paraffin embedded tissue block Transport Temperature Room temperature Specimen Stability Room temperature: Indefinite Refrigerated: Indefinite Frozen: Unacceptable Reject Criteria Received frozen